##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SAMPLE_BAM_2.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24890
Total Bases	2.5 Mbp
Sequences flagged as poor quality	0
Sequence length	101
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.57492969063881	33.0	30.0	34.0	22.0	35.0
2	30.69433507432704	34.0	31.0	34.0	26.0	35.0
3	30.848734431498595	34.0	31.0	34.0	26.0	35.0
4	32.70747288067497	35.0	33.0	37.0	27.0	37.0
5	32.74596223382885	35.0	33.0	37.0	27.0	37.0
6	32.861872237846526	35.0	33.0	37.0	27.0	37.0
7	32.93921253515468	35.0	33.0	37.0	27.0	37.0
8	33.00514262756127	35.0	33.0	37.0	27.0	37.0
9	33.8412213740458	35.0	34.0	39.0	28.0	39.0
10-11	33.829349136199276	35.0	33.5	39.0	27.0	39.0
12-13	33.921916432302126	35.0	34.0	39.0	27.0	39.0
14-15	34.714564081960624	35.0	34.0	40.0	27.5	41.0
16-17	34.80435918039373	35.0	34.0	40.0	27.5	41.0
18-19	34.94558055443953	35.5	34.0	40.0	29.0	41.0
20-21	35.046484531940536	36.0	34.0	40.0	29.0	41.0
22-23	35.09608276416231	36.0	34.0	40.0	29.0	41.0
24-25	35.23935315387706	36.0	34.0	40.0	29.0	41.0
26-27	35.36805946163118	36.5	34.0	40.0	29.0	41.0
28-29	35.50271193250302	37.0	34.0	40.0	29.0	41.0
30-31	35.60421856167135	37.0	34.5	40.0	30.0	41.0
32-33	35.74469666532744	37.5	35.0	40.0	29.5	41.0
34-35	36.02165528324629	38.0	35.0	40.0	30.0	41.0
36-37	36.184612294094016	38.0	35.0	40.5	30.0	41.0
38-39	36.30578545600643	38.0	35.0	41.0	30.0	41.0
40-41	36.378987545198875	39.0	35.0	41.0	30.0	41.0
42-43	36.34829248694255	39.0	35.0	41.0	30.0	41.0
44-45	36.36357975090398	39.0	35.0	41.0	30.0	41.0
46-47	36.234371233427076	39.0	35.0	41.0	29.5	41.0
48-49	36.107211731619124	38.5	35.0	40.0	29.0	41.0
50-51	35.15144636400161	37.5	33.5	39.5	27.5	40.5
52-53	35.92280032141423	38.0	34.5	40.0	29.0	41.0
54-55	36.225873844917636	39.0	35.0	40.0	29.0	41.0
56-57	36.31076737645641	39.0	35.0	41.0	30.0	41.0
58-59	36.4865608678184	39.0	35.0	41.0	30.0	41.0
60-61	36.530192848533545	39.0	35.0	41.0	30.0	41.0
62-63	36.54286862193652	39.0	35.0	41.0	30.0	41.0
64-65	36.38429087987143	38.5	35.0	41.0	30.0	41.0
66-67	36.25743270389715	38.0	35.0	41.0	30.0	41.0
68-69	36.083748493370834	38.0	35.0	40.0	30.0	41.0
70-71	35.820972278023305	37.5	35.0	40.0	30.0	41.0
72-73	35.67119325030133	37.0	34.5	40.0	30.0	41.0
74-75	35.54465648854962	37.0	34.0	40.0	29.5	41.0
76-77	35.393893129770994	37.0	34.0	40.0	29.0	41.0
78-79	35.25562474889514	36.0	34.0	40.0	29.0	41.0
80-81	35.11412213740458	36.0	34.0	40.0	29.0	41.0
82-83	35.06054640417838	36.0	34.0	40.0	29.0	41.0
84-85	34.89851345922057	36.0	34.0	40.0	28.5	41.0
86-87	34.81500602651667	35.0	34.0	40.0	28.5	41.0
88-89	34.376677380474085	35.0	34.0	39.5	27.5	40.0
90-91	33.95940136601045	35.0	34.0	39.0	27.0	39.0
92-93	33.93465247087184	35.0	34.0	39.0	28.0	39.0
94-95	33.07711932503013	35.0	34.0	37.0	28.0	37.0
96-97	32.921273603856974	35.0	33.0	37.0	27.5	37.0
98-99	31.88836882282041	34.5	32.0	35.5	26.5	36.0
100-101	30.31892326235436	33.5	30.5	34.0	24.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9.0
3	6.0
4	4.0
5	8.0
6	10.0
7	11.0
8	21.0
9	27.0
10	27.0
11	43.0
12	44.0
13	46.0
14	53.0
15	51.0
16	62.0
17	64.0
18	68.0
19	80.0
20	91.0
21	105.0
22	126.0
23	122.0
24	165.0
25	185.0
26	243.0
27	292.0
28	380.0
29	498.0
30	499.0
31	705.0
32	919.0
33	1199.0
34	1717.0
35	2388.0
36	3719.0
37	5554.0
38	4794.0
39	555.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.828043390920048	22.635596625150665	27.284049819204498	28.252310164724786
2	27.653676175170748	23.587786259541986	25.150662916834072	23.607874648453194
3	26.476496584973887	22.17758135797509	25.34351145038168	26.002410606669347
4	25.994375251104863	22.511048613901167	24.82523101647248	26.66934511852149
5	25.869827239855365	21.912414624347125	26.179188429087986	26.038569706709524
6	26.155082362394538	22.04901566894335	24.86942547207714	26.92647649658497
7	26.53676175170751	21.96062675773403	25.58457211731619	25.91803937324227
8	26.287665729208516	21.53073523503415	25.87786259541985	26.303736440337484
9	26.05865809562073	21.651265568501408	26.09883487344315	26.19124146243471
10-11	26.14503816793893	22.02691844114102	25.552430695058252	26.275612695861795
12-13	25.845721173161913	22.205705102450786	25.69304941743672	26.255524306950583
14-15	25.869827239855365	21.8521494576135	25.87786259541985	26.400160707111294
16-17	26.313780634793087	21.978706307754116	25.789473684210527	25.91803937324227
18-19	25.924065889915624	21.898352752109282	25.373644033748494	26.803937324226595
20-21	25.94616311771796	21.900361591000404	25.829650462032944	26.323824829248693
22-23	26.404178384893534	22.278023302531135	25.208919244676576	26.108879067898755
24-25	26.331860184813177	21.862193652069102	25.58256327842507	26.223382884692647
26-27	26.02852551225392	22.448774608276416	25.122539172358376	26.400160707111294
28-29	26.458417034953797	22.36440337484934	24.965849738850945	26.211329851345923
30-31	26.741663318601848	22.141422257934913	24.903575733226198	26.21333869023704
32-33	26.55685014061872	22.398553635998393	25.06830052229811	25.976295701084773
34-35	26.313780634793087	22.253917235837687	25.331458417034952	26.10084371233427
36-37	26.106870229007633	22.490960224989955	25.29529931699478	26.106870229007633
38-39	26.35797509039775	22.25994375251105	25.208919244676576	26.17316191241462
40-41	26.000401767778225	22.43069505825633	25.25512253917236	26.313780634793087
42-43	26.223382884692647	22.036962635596623	25.214945761349938	26.52470871836079
44-45	25.763358778625957	22.163519485737243	25.293290478103657	26.779831257533143
46-47	25.9501807955002	21.850140618722378	25.245078344716752	26.954600241060668
48-49	26.329851345922055	21.83607874648453	24.797107271996786	27.036962635596623
50-51	25.53033346725593	22.038971474487745	24.91161108879068	27.519083969465647
52-53	26.434310968260345	22.24186420249096	24.533949377259944	26.78987545198875
54-55	25.8256327842507	21.898352752109282	25.208919244676576	27.067095218963438
56-57	25.335476094817196	22.31016472478907	25.008035355564484	27.34632382482925
58-59	25.920048212133384	22.28806749698674	24.50381679389313	27.28806749698674
60-61	25.719164323021293	21.908396946564885	24.90759341100844	27.464845319405384
62-63	25.932101245480112	22.683808758537566	24.483728404981917	26.900361591000397
64-65	26.20329449578144	22.484933708316593	24.87143431096826	26.440337484933707
66-67	26.18521494576135	22.3222177581358	24.620329449578144	26.872237846524712
68-69	25.938127762153474	21.926476496584975	25.172760144636403	26.96263559662515
70-71	26.707513057452793	22.298111691442347	24.562073121735637	26.432302129369223
72-73	26.096826034552027	22.302129369224588	24.728806749698673	26.872237846524712
74-75	26.675371635194857	22.123342707914826	24.664523905182804	26.53676175170751
76-77	26.757734029730816	22.488951386098837	24.35114503816794	26.402169546002412
78-79	27.03093611892326	22.290076335877863	24.69666532744074	25.982322217758135
80-81	26.484531940538368	22.649658497388508	24.236641221374043	26.629168340699078
82-83	26.667336279630373	22.643631980715146	24.483728404981917	26.205303334672557
84-85	26.44234632382483	22.442748091603054	24.797107271996786	26.31779831257533
86-87	26.61309762957011	22.187625552430696	24.431498593812776	26.767778224186422
88-89	26.896343913218164	22.66974688629972	24.3471273603857	26.086781840096425
90-91	26.255524306950583	22.46082764162314	24.28887103254319	26.994777018883088
92-93	26.84813177983126	22.9750903977501	23.967456809963842	26.2093210124548
94-95	26.932503013258334	22.641623141824024	23.53555644837284	26.8903173965448
96-97	27.203696263559664	22.58537565287264	24.039775010044195	26.171153073523502
98-99	27.101245480112496	22.531137002812372	24.015668943350743	26.35194857372439
100-101	27.085174768983528	22.94696665327441	25.38770590598634	24.580152671755727
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	0.5
24	1.0
25	1.5
26	1.5
27	2.0
28	2.5
29	4.0
30	8.5
31	13.5
32	22.5
33	43.5
34	84.5
35	163.5
36	252.5
37	363.0
38	462.0
39	525.0
40	531.5
41	530.5
42	526.0
43	488.5
44	500.0
45	547.5
46	581.0
47	617.0
48	735.5
49	880.0
50	969.5
51	1069.5
52	1130.5
53	1118.0
54	1060.5
55	1076.0
56	1149.0
57	1119.0
58	1104.0
59	1045.0
60	930.5
61	870.5
62	795.5
63	659.5
64	548.5
65	464.5
66	399.0
67	354.0
68	280.5
69	226.0
70	182.0
71	134.0
72	105.0
73	77.5
74	48.0
75	30.0
76	20.0
77	12.5
78	8.0
79	5.5
80	4.0
81	3.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	24890.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.88228204098031
#Duplication Level	Percentage of total
1	76.82201687424669
2	16.022498995580555
3	4.423463238248292
4	1.3820811570912013
5	0.622740056247489
6	0.3374849337083166
7	0.08437123342707915
8	0.19284853354760947
9	0.07231820008035356
>10	0.040176777822418644
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
2	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
3	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
4	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
5	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
6	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
7	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
8	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
9	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
10-11	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
12-13	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
14-15	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
16-17	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
18-19	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
20-21	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
22-23	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
24-25	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
26-27	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
28-29	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
30-31	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
32-33	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
34-35	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
36-37	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
38-39	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
40-41	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
42-43	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
44-45	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
46-47	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
48-49	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
50-51	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
52-53	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
54-55	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
56-57	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
58-59	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
60-61	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
62-63	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
64-65	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
66-67	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
68-69	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
70-71	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
72-73	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
74-75	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
76-77	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
78-79	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
80-81	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
82-83	0.0	0.0	0.0	0.0	0.0	0.004017677782241864
84-85	0.01807955002008839	0.0	0.0	0.0	0.0	0.004017677782241864
86-87	0.052229811169144234	0.0	0.0	0.0	0.0	0.004017677782241864
88-89	0.13660104459622338	0.0	0.0	0.0	0.0	0.004017677782241864
90	0.22097227802330252	0.0	0.0	0.0	0.0	0.004017677782241864
>>END_MODULE
