##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SAMPLE_FASTQ_2.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43392
Total Bases	4.3 Mbp
Sequences flagged as poor quality	0
Sequence length	101
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.239652470501476	32.0	30.0	34.0	19.0	34.0
2	30.324875553097346	34.0	31.0	34.0	25.0	35.0
3	30.471169800884955	34.0	31.0	34.0	25.0	35.0
4	32.406826143067846	35.0	33.0	37.0	25.0	37.0
5	32.46997142330383	35.0	33.0	37.0	26.0	37.0
6	32.5975755899705	35.0	33.0	37.0	26.0	37.0
7	32.697087020648965	35.0	33.0	37.0	27.0	37.0
8	32.76998064159292	35.0	33.0	37.0	27.0	37.0
9	33.643229166666664	35.0	33.0	39.0	27.0	39.0
10-11	33.65741380899705	35.0	33.0	39.0	27.0	39.0
12-13	33.7835776179941	35.0	33.0	39.0	27.0	39.0
14-15	34.57738753687316	35.0	34.0	40.0	27.0	41.0
16-17	34.671598451327434	35.0	34.0	40.0	27.0	41.0
18-19	34.81902193952802	35.5	34.0	40.0	27.0	41.0
20-21	34.8888043879056	36.0	34.0	40.0	27.0	41.0
22-23	34.93060932890856	36.0	34.0	40.0	28.5	41.0
24-25	35.07814804572271	36.0	34.0	40.0	28.5	41.0
26-27	35.21038440265487	36.0	34.0	40.0	29.0	41.0
28-29	35.30194505899705	37.0	34.0	40.0	29.0	41.0
30-31	35.421367994100294	37.0	34.0	40.0	29.0	41.0
32-33	35.580925055309734	37.0	34.0	40.0	29.5	41.0
34-35	35.814274520648965	37.5	35.0	40.0	30.0	41.0
36-37	35.983533831120944	38.0	35.0	40.0	30.0	41.0
38-39	36.08902562684366	38.0	35.0	40.5	30.0	41.0
40-41	36.181750092182895	38.0	35.0	41.0	30.0	41.0
42-43	36.182625829646014	38.0	35.0	41.0	30.0	41.0
44-45	36.16353244837758	38.0	35.0	41.0	29.5	41.0
46-47	36.032586651917406	38.0	35.0	40.0	29.0	41.0
48-49	35.90020049778761	38.0	34.5	40.0	28.0	41.0
50-51	34.974257927728615	37.5	33.5	39.5	27.0	40.5
52-53	35.72202249262537	38.0	34.0	40.0	28.0	41.0
54-55	36.040237831858406	38.0	35.0	40.0	28.5	41.0
56-57	36.18007927728614	38.5	35.0	40.5	29.0	41.0
58-59	36.36444505899705	39.0	35.0	41.0	30.0	41.0
60-61	36.39038301991151	39.0	35.0	41.0	30.0	41.0
62-63	36.37708563790561	38.5	35.0	41.0	30.0	41.0
64-65	36.19891915560472	38.0	35.0	40.0	30.0	41.0
66-67	36.0419777839233	38.0	35.0	40.0	30.0	41.0
68-69	35.85221930309734	37.5	35.0	40.0	30.0	41.0
70-71	35.57789454277286	37.0	34.0	40.0	29.5	41.0
72-73	35.4375	37.0	34.0	40.0	29.0	41.0
74-75	35.27972898230088	36.5	34.0	40.0	29.0	41.0
76-77	35.134080014749266	36.0	34.0	40.0	29.0	41.0
78-79	34.9858729719764	36.0	34.0	40.0	29.0	41.0
80-81	34.8210614859882	35.5	34.0	40.0	28.0	41.0
82-83	34.77100617625369	35.0	34.0	40.0	28.0	41.0
84-85	34.628134218289084	35.0	34.0	40.0	27.0	41.0
86-87	34.55679618362832	35.0	34.0	40.0	27.0	41.0
88-89	34.1357277839233	35.0	33.5	39.5	27.0	40.0
90-91	33.70306738569322	35.0	33.0	39.0	27.0	39.0
92-93	33.68801852876106	35.0	33.0	39.0	27.0	39.0
94-95	32.836905420353986	35.0	33.0	37.0	27.0	37.0
96-97	32.67332688053097	35.0	33.0	37.0	27.0	37.0
98-99	31.630554019174042	34.5	32.0	35.5	25.5	36.0
100-101	30.042127581120944	33.5	30.5	34.0	22.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13.0
3	10.0
4	7.0
5	12.0
6	15.0
7	17.0
8	34.0
9	40.0
10	46.0
11	70.0
12	86.0
13	89.0
14	86.0
15	88.0
16	100.0
17	109.0
18	129.0
19	155.0
20	169.0
21	201.0
22	229.0
23	248.0
24	324.0
25	377.0
26	481.0
27	569.0
28	706.0
29	894.0
30	1007.0
31	1362.0
32	1713.0
33	2225.0
34	3120.0
35	4247.0
36	6648.0
37	9871.0
38	7254.0
39	641.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.44865412979351	22.273691002949853	29.452433628318587	26.825221238938052
2	27.449760324483773	23.343012536873157	26.608591445427727	22.59863569321534
3	26.216814159292035	22.02940634218289	27.484328908554573	24.2694505899705
4	25.81120943952802	21.868086283185843	26.864398967551622	25.45630530973451
5	25.36181784660767	21.902654867256636	28.546736725663717	24.188790560471976
6	25.58305678466077	21.414085545722713	27.37370943952802	25.629148230088493
7	26.34356563421829	21.492441002949853	27.44745575221239	24.71653761061947
8	26.55558628318584	20.980825958702066	27.210084808259587	25.253502949852507
9	25.550792772861357	21.460176991150444	27.883019911504427	25.106010324483773
10-11	26.335499631268437	21.58808075221239	26.925470132743364	25.150949483775808
12-13	26.002488938053098	21.783969395280238	26.938145280235986	25.275396386430675
14-15	25.950636061946902	21.570796460176993	26.840200958702066	25.638366519174046
16-17	26.534845132743364	21.604212758112094	26.49566740412979	25.365274705014752
18-19	26.46340339233038	21.425608407079647	26.20759587020649	25.90339233038348
20-21	26.964647861356934	21.24009033923304	26.378134218289084	25.417127581120948
22-23	27.160536504424783	21.67334992625369	25.627995943952804	25.538117625368734
24-25	27.08218104719764	21.697547935103245	25.507005899705014	25.713265117994098
26-27	26.534845132743364	22.017883480825958	24.998847713864304	26.448423672566374
28-29	27.222759955752213	21.84964970501475	25.046091445427727	25.88149889380531
30-31	27.43362831858407	21.196303466076696	24.90666482300885	26.46340339233038
32-33	27.288440265486724	21.969487463126843	24.882466814159294	25.859605457227136
34-35	26.744561209439528	21.80355825958702	25.07605088495575	26.3758296460177
36-37	26.169570427728612	21.938375737463126	25.250046091445427	26.642007743362832
38-39	26.492210545722717	21.8922842920354	24.567892699115042	27.047612463126843
40-41	26.162656710914455	21.80586283185841	24.99654314159292	27.034937315634217
42-43	26.39772308259587	20.950866519174042	24.975801991150444	27.675608407079643
44-45	25.448239306784657	21.281572640117993	25.07259402654867	28.19759402654867
46-47	26.065864675516227	21.06839970501475	24.479166666666664	28.38656895280236
48-49	25.707503687315636	21.311532079646017	24.10352138643068	28.87744284660767
50-51	25.078355457227136	21.425608407079647	24.501060103244836	28.994976032448378
52-53	26.49336283185841	21.46939528023599	23.606886061946902	28.430355825958703
54-55	26.07277839233038	20.873663348082598	24.68773045722714	28.365827802359885
56-57	25.141731194690266	21.568491887905605	24.4054203539823	28.884356563421832
58-59	25.518528761061948	21.547750737463126	23.53429203539823	29.399428466076692
60-61	25.285766961651916	21.46248156342183	23.881130162241888	29.370621312684364
62-63	25.62338679941003	22.03286320058997	23.654129793510325	28.689620206489675
64-65	25.765117994100294	21.974096607669615	24.374308628318584	27.886476769911507
66-67	25.94026548672566	21.500507005899706	24.246404867256636	28.312822640117997
68-69	25.533508480825954	21.192846607669615	24.469948377581122	28.803696533923308
70-71	26.779129793510325	21.84619284660767	23.33379424778761	28.040883112094395
72-73	25.793925147492626	21.88997971976401	23.96063790560472	28.355457227138643
74-75	25.9852046460177	21.485527286135696	24.277516592920353	28.25175147492625
76-77	26.011707227138643	22.08010693215339	23.705982669616517	28.202203171091444
78-79	27.18127765486726	21.76092367256637	23.596515486725664	27.461283185840706
80-81	26.332042772861357	22.112370943952804	23.637997787610622	27.917588495575217
82-83	26.084301253687315	22.199944690265486	23.821211283185843	27.894542772861357
84-85	26.40348451327434	22.377396755162245	23.363753687315633	27.855365044247787
86-87	26.27097160766962	22.302498156342182	23.313053097345133	28.113477138643066
88-89	26.107346976401182	23.164408185840706	23.10103244837758	27.627212389380528
90-91	25.746681415929203	22.75995575221239	23.3015302359882	28.19183259587021
92-93	26.103890117994098	23.30844395280236	22.721930309734514	27.865735619469028
94-95	26.5625	22.929341814159294	22.331305309734514	28.176852876106196
96-97	26.63163716814159	22.948930678466077	22.930494100294986	27.488938053097346
98-99	26.497971976401182	22.811808628318584	22.88555494100295	27.804664454277283
100-101	26.43574852507375	23.431738569321535	24.427313790560472	25.70519911504425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.0
23	1.0
24	1.5
25	2.0
26	2.5
27	4.5
28	5.5
29	6.5
30	13.5
31	24.5
32	38.5
33	61.5
34	114.0
35	212.5
36	323.0
37	452.0
38	563.5
39	643.5
40	667.5
41	674.5
42	658.5
43	607.0
44	629.5
45	708.0
46	809.5
47	984.5
48	1321.5
49	1715.0
50	1977.5
51	2147.5
52	2212.0
53	2122.0
54	2005.5
55	2045.0
56	2171.5
57	2224.0
58	2285.0
59	2261.0
60	2060.0
61	1785.0
62	1543.0
63	1264.5
64	962.0
65	743.0
66	590.5
67	466.5
68	343.5
69	261.5
70	199.5
71	143.0
72	110.0
73	81.5
74	51.0
75	33.0
76	22.5
77	12.5
78	8.0
79	5.5
80	4.0
81	3.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	43392.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.17772861356931
#Duplication Level	Percentage of total
1	80.77756268436578
2	13.555494100294984
3	3.4914269911504423
4	1.1246312684365782
5	0.47243731563421826
6	0.27654867256637167
7	0.09679203539823009
8	0.11061946902654868
9	0.020741150442477877
>10	0.07374631268436578
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
2	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
3	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
4	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
5	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
6	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
7	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
8	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
9	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
10-11	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
12-13	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
14-15	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
16-17	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
18-19	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
20-21	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
22-23	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
24-25	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
26-27	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
28-29	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
30-31	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
32-33	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
34-35	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
36-37	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
38-39	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
40-41	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
42-43	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
44-45	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
46-47	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
48-49	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
50-51	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
52-53	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
54-55	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
56-57	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
58-59	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
60-61	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
62-63	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
64-65	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
66-67	0.0	0.0	0.0	0.0	0.004609144542772861	0.0023045722713864306
68-69	0.0	0.0	0.0	0.0	0.005761430678466076	0.0023045722713864306
70-71	0.0	0.0	0.0	0.0	0.006913716814159292	0.0023045722713864306
72-73	0.0	0.0	0.0	0.0	0.006913716814159292	0.0023045722713864306
74-75	0.0	0.0	0.0	0.0	0.010370575221238937	0.0023045722713864306
76-77	0.0	0.0	0.0	0.0	0.01728429203539823	0.0023045722713864306
78-79	0.0	0.0	0.0	0.0	0.035720870206489674	0.0023045722713864306
80-81	0.0	0.0	0.0	0.0	0.05185287610619469	0.0023045722713864306
82-83	0.005761430678466076	0.0	0.0	0.0	0.07028945427728614	0.0023045722713864306
84-85	0.023045722713864306	0.0	0.0	0.0	0.11177175516224189	0.0023045722713864306
86-87	0.04378687315634218	0.0	0.0	0.0	0.1647769174041298	0.0023045722713864306
88-89	0.09679203539823009	0.0	0.0	0.0	0.1889749262536873	0.0023045722713864306
90	0.15210176991150443	0.0	0.0	0.0	0.21202064896755163	0.0023045722713864306
>>END_MODULE
